whole brain cdna (TaKaRa)

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TaKaRa whole brain cdna
Whole Brain Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/whole+brain+cdna/Human+Brain%2C+Whole+QUICK-Clone+cDNA/pmc12984142-228-14-17
Average 94 stars, based on 545 article reviews

whole brain cdna - by Bioz Stars, 2026-10

94/100 stars

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Amplification:

Article Title: Ancient Origins of RGK Protein Function: Modulation of Voltage-Gated Calcium Channels Preceded the Protostome and Deuterostome Split
Article Snippet: .. Open reading frames for human Rit1 (AF084462) and Rit2 (NM_002930) were amplified from human whole brain cDNA (Clontech) and cloned into pcDNA3.1 at the KpnI / XhoI sites for Rit1, or the BamHI / XhoI sites for Rit2. ..

Article Title: Genetically Encoded CB 2 R-Based Fluorescent Sensor Enables Rapid Screening and Functional Assessment of Cannabinoid Modulators
Article Snippet: .. Briefly the open reading frame of hCB2 ( NM_001841 ) was amplified from human whole brain cDNA (Takara) and inserted in place of the hCB1 sensor portion of eCB2.0 using the In Fusion cloning system (Takara, San Jose, CA, USA). ..

Article Title: Genetically Encoded CB2R-Based Fluorescent Sensor Enables Rapid Screening and Functional Assessment of Cannabinoid Modulators
Article Snippet: .. Briefly the open reading frame of hCB2 (NM_001841) was amplified from human whole brain cDNA (Takara) and inserted in place of the hCB1 sensor portion of eCB2.0 using the In Fusion cloning system (Takara, San Jose, CA, USA). ..

Clone Assay:

Derivative Assay:

Article Title: A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo
Article Snippet: .. Templates for assembly were derived from human whole-brain cDNA (Takara) for all cDNAs, except for the hGNB1 and hGNG2 Venus fusions which were a generous gift from Dr. Nevin Lambert (Augusta University). .. Approximately 24 hours after transfection, the cells were harvested with 10 mM EDTA in phosphate-buffered saline (PBS, pH 7.2), pelleted, and then resuspended in Dulbecco’s modified PBS (Life Technologies) without Ca 2+ or Mg 2+ .

Article Title: A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo.
Article Snippet: .. Templates for assembly were derived from human whole-brain cDNA (Takara) for all cDNA, except for those for hGNB1 and hGNG2 Venus fusions, which were a generous gift from N. Lambert (Augusta University). .. Approximately 24 h after transfection, cells were collected using 10 mM EDTA in PBS (pH 7.2), pelleted and then resuspended in Dulbecco’s modified PBS (Life Technologies) without Ca2+ or Mg2+.

Plasmid Preparation:

Article Title: 8-Bicycloalkyl-CPFPX derivatives as potent and selective tools for in vivo imaging of the A 1 adenosine receptor.
Article Snippet: .. Human adenosine receptors ADORA1 and ADORA2A from human whole brain cDNA (Clontech) cloned into a pcDNA3.1+ vector (Invitrogen) at EcoRI (5′), XhoI (3′) for ADORA1 and BamHI(5′), XhoI (3′) for ADORA2A were purchased from The Missouri S&T cDNA Resource Center, USA. .. The plate was analyzed with a phosphor imager (BAS 5000, Fujifilm, Düsseldorf, Germany) and tracer binding was quantified via 2D densitometry (AIDA Image Analysis software V5.1).

Cloning:

Article Title: Genetically Encoded CB2R-Based Fluorescent Sensor Enables Rapid Screening and Functional Assessment of Cannabinoid Modulators
Article Snippet: .. Briefly the open reading frame of hCB2 (NM_001841) was amplified from human whole brain cDNA (Takara) and inserted in place of the hCB1 sensor portion of eCB2.0 using the In Fusion cloning system (Takara, San Jose, CA, USA). ..

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